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The Tyrannosaurus rex nest is found by Roland Tembo and Ajay Sidhu in Roland’s quest to hunt a Tyrannosaurus buck while he is on the InGen Harvest Operation led by Peter Ludlow in 1997. The nest is occupied by one Tyrannosaurus infant and several carcasses (one of which the infant was feeding on) in various states of decay. The area was buzzing with swarms of flies attracted to the stench of the rotting meat. Located within a rocky pit on the Northeastern side of the island, the nest was well hidden and secluded from invaders. According to Roland the nest was located upwind, which was why he planned to use the infant to trap the Tyrannosaurus in a locale that gave Roland and Ajay a better advantage. Had he stayed, the Tyrannosaurus parents would have known the two hunters were there before Roland or Ajay ever had a chance to know of the Tyrannosaurus presence. The infant was estimated by Roland to be only a few weeks old and having never left the nest. This would confirm Dr. Sarah Harding’s earlier hypothesis of Tyrannosaurus being nurturing parents, which was one of the many things Sarah wanted to find proof of during her time on Isla Sorna.
In Michael Crichton's 1995 novel, a Biosyn team led by Dr. Lewis Dodgson encounter the Tyrannosaurus nest on Isla Sorna, hoping to steal eggs. The nest is located near the center of the island, south of the main compound. The parents fiercely gaurded the nest, even going so far as to starve themselves to protect it. When Dodgson approached the nest with his team, he used a sonic device to keep the parents away while one of his associates attempted to steal an already hatched infant. When the device malfunctioned, the Biosyn team had to flee in a hurry, breaking one of the infant's legs in the process. The next day, the Tyrannosaurus parents eventually find Dodgson, and bring him to the nest, where he is fed to the Tyrannosaurus infants.